Oxford Nanopore Technologies Ltd  

The GridION Mk1 is ideal for labs with multiple projects: the instrument provides the advantages of long-read nanopore sequencing with the added flexibility to run up to five MinION or Flongle Flow Cells simultaneously or individually. The GridION also allows users to offer nanopore sequencing as a service.

With an output of up to 150 Gb (from five MinION Flow Cells), the GridION is well suited to human whole-genome and transcriptome sequencing. Nanopore long DNA sequencing reads enable users to accurately assemble genomes, fully characterise structural variants, resolve challenging repetitive regions, and perform haplotype phasing. Nanopore full-length RNA sequencing allows unambiguous identification of splice variants and gene fusions, as well as accurate transcript quantification. With the unique ability of Nanopore technology to directly sequence native DNA and RNA, base modifications can be detected alongside nucleotide sequence, enabling in-depth interrogation of your sequencing data.

MinION Mk1C is designed to enable rapid DNA/RNA sequencing in any environment, with simple hardware and 4G connectivity. We have designed it to support our goal of enabling the analysis of any living thing, by any person, in any environment.

MinION Mk1C can run MinION flow cells, or using the Flongle adapter it can run smaller Flongle flow cells.

Choose Mk1C if:

• You would like a solution for long read, high fidelity, direct DNA or RNA sequencing
• MinION flow cells:  With powerful throughput (as much as 30Gb per flow cell) and excellent value (from $500 per flow cell)
• Flongle flow cells:  For rapid, smaller tests with low cost per flow cell (~$100 per flow cell)
• You would like a simple hardware solution that does not require laptop or computer to run the device, manage the experiment or perform analysis (all integrated into Mk1C)
• You would like to take sequencing out of a lab environment and into the field, with a connected device.

With an output of up to 1.8 Gb of sequencing data, Flongle is ideal for the analysis of smaller samples, such as amplicons and targeted regions, as well as for rapid quality checking of a sample prior to sequencing on higher output devices, such as the MinION, GridION, or PromethION. Flongle uses the same core Nanopore technology as these other platforms, offering direct DNA or RNA analysis, simple library preparation, real-time data streaming, and long and ultra-long sequencing reads.

The 16S rRNA gene is present in all bacteria and archaea and serves as a means of identification based on the individual sequence allowing comparison of taxonomic diversity within a sample or identifying the presence of specific bacteria of interest. Oxford Nanopore Technologies’ 16S kit targets the whole gene, not just the hypervariable regions so there is more taxonomic context with which to assign a classification to the read, improving precision in metagenomic species identification.

Why do 16S sequencing using Oxford Nanopore’s 16S Barcoding kit? Oxford Nanopore devices can sequence long reads, so targeting the whole 16S rRNA gene with a single amplicon is easy and simple.

• Prepare, sequence, analyse: an end to end workflow to assess metagenomic diversity.
• PCR Primers are optimised for sequencing on Oxford Nanopore devices.
• No optimisation of multiple primers to cover the hypervariable regions is required
• Easy amplification of target within a background of host
• Quick workflow –5 minutes post PCR to prep
• Up to 24 individual barcodes available, reducing costs per sample.
• Simple to use analysis workflow to provide a report of what your microbiome contains.

This kit is recommended for users who

• wish to multiplex samples to reduce price per sample
• want to do 16S sequencing
• are interested in genus level bacterial identification

Barcoding or multiplexing is useful when the amount of data required per sample is less than the total amount of data that can be generated from a single flow cell: it allows a user to pool multiple samples and sequence them together making more efficient use of the flow cell. 

The 16S Barcoding Kit offers a method of amplifying and barcoding the ~1500 bp 16S rRNA gene from multiple samples and sequencing them together. By narrowing down to a specific region of interest, a user can see all the organisms in the sample without sequencing unnecessary regions of the genome, making the identification quicker and more economical. 

The kit is supported by the EPI2ME 16S-BLAST workflow, which can be used to analyse data from the 16S protocol. Deconvolution of barcoded sequencing data is supported by Guppy and EPI2ME which classify the barcode sequence and sort reads into corresponding folders.

PromethION provides real-time, on-demand nanopore sequencing; users may choose to run as many or as few of the 24 (P24) or 48 (P48) flow cells as required, at any time.  With each flow cell capable of producing >200 Gb of data, the PromethION outputs terabases of sequence data, bringing the benefits of long-read nanopore sequencing to large-scale population studies. With flow cell pricing from $625, researchers can cost-effectively sequence 10s to 1,000s of samples.

Long-read DNA sequencing with the PromethION enables users to accurately assemble whole human genomes, comprehensively analyse structural variation, resolve challenging repetitive regions, and perform haplotype phasing. With full-length RNA transcripts, extensively characterise the transcriptome. Due to the unique ability of Nanopore technology to directly sequence native DNA and RNA, base modifications can be detected alongside nucleotide sequence, enabling in-depth interrogation of your sequencing data.